![]() It is important to properly arrange and secure materials in the chamber by using an autoclave basket to ensure that they are evenly exposed to the steam.Never put materials that cannot be autoclaved, for instance, flammable materials, radioactive materials, and plastics that are not intended for sterilization. ![]() ![]() Materials must be placed in the sterilization chamber and exposed to steam at high temperatures and pressures.The proper use of autoclavesīefore any sterilization process takes place, ensure the inside chamber is clean and the water inside the autoclave is sufficient for the process. To be effectively eliminating the microbes, the autoclave usually operates at 121ᵒC / 15psi for 20 minutes as most of the bacteria cannot withstand this condition. The temperature, pressure, and sterilization time are carefully controlled to ensure that materials are properly sterilized. The principles of autoclavesĪutoclaves use high-pressure steam to kill microorganisms (bacteria, viruses, and fungi), through the process of steam sterilization. In this article, we will cover the autoclave principles, proper use, and maintenance. Autoclaves are essential in maintaining aseptic techniques and reducing the risk of contamination and are important in healthcare, research, and the food processing industry. This process is known as steam sterilization and involves exposing materials to steam at high temperatures for a specific period of time aiming to kill all microorganisms inside the machine. All rights reserved.An autoclave is a specialized machine used in laboratories, hospitals, and other healthcare settings to sterilize equipment and materials through the use of high-pressure steam for a specific duration. Possible improvements include renewed emphasis during initial nurse education, greater opportunity for updating knowledge and skills post-qualification, and audit of practice.Īseptic technique Content analysis Infection prevention and control Nursing.Ĭopyright © 2018 Association for Professionals in Infection Control and Epidemiology, Inc. Further studies should establish the generalizability of the study findings. Nurses' understanding of the principles of asepsis could be improved. This knowledge gap potentially places patients at risk. ![]() Nurses' understanding of aseptic technique and the concepts of sterility and cleanliness is inadequate, a finding in line with results of previous studies. Qualitative analysis confirmed a lack of clarity about the meaning of aseptic technique. Additionally, 72% reported that they not had received training for at least 5 years 92% were confident of their ability to apply aseptic technique and 90% reported that they had not been reassessed since their initial training. The related concepts of cleanliness and sterilization were frequently confused with one another. Of those responding, 65% of nurses described aseptic technique in terms of the procedure used to undertake it, and 46% understood the principles of asepsis. In many countries, aseptic procedures are undertaken by nurses in the general ward setting, but variation in practice has been reported, and evidence indicates that the principles underpinning aseptic technique are not well understood.Ī survey was conducted, employing a brief, purpose-designed, self-reported questionnaire.
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